Mouse GLUT1/Glucose Transporter 1 ELISA Kit

Assay Genie offers a high-quality sandwich ELISA kit designed for the quantitative detection of mouse Glucose Transporter 1 (GLUT1) in research samples. The kit uses a double antibody sandwich format with colorimetric detection and is reactive with mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

With a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL, the kit is suited to studies of glucose metabolism. GLUT1 is a key transporter protein involved in glucose uptake and plays a critical role in maintaining glucose homeostasis, with dysregulation associated with metabolic disorders such as diabetes and cancer. The kit is stored at 2-8°C for 12 months.

Linearity ranges from 88-105% in serum, 82-100% in EDTA plasma, and 82-99% in heparin plasma across tested dilutions. Recovery ranged from 89-104% in serum (average 94%), 87-105% in EDTA plasma (average 95%), and 86-104% in heparin plasma (average 96%). The assay procedure involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-streptavidin binding, TMB substrate color development, and absorbance measurement at 450 nm.