Mouse Glucosylceramidase/GBA ELISA Kit

Designed for the precise measurement of glucosylceramidase (GBA) levels in mouse samples, this Sandwich ELISA from Assay Genie uses a double-antibody format with colorimetric detection. Glucosylceramidase is a key lysosomal enzyme involved in lipid metabolism, and dysregulation of this enzyme has been linked to lysosomal storage disorders, including Gaucher disease. The kit is intended for research use, supporting investigations into the pathophysiology of these conditions and the development of potential therapeutic interventions.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 0.188 ng/mL and a detection range of 0.313–20 ng/mL. Standards, samples and controls are incubated on a pre-coated plate at 37°C for 90 minutes, followed by a biotin-labelled detection antibody (60 minutes at 37°C), HRP-streptavidin binding (30 minutes at 37°C), TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm after addition of stop solution. Kit components are stored at 2–8°C for 12 months. Synonyms recognised by the antibody include Gba, Glucosylceramidase, Imiglucerase, D-glucosyl-N-acylsphingosine glucohydrolase, Beta-glucocerebrosidase, Beta-GC, Acid beta-glucosidase, Alglucerase, GC, GLUC and GBA1 (UniProt P17439).

Reported linearity across 1:2, 1:4 and 1:8 dilutions is 89–105%, 85–102% and 85–101% for serum (n=5); 90–99%, 83–97% and 84–100% for EDTA plasma (n=5); and 80–94%, 83–99% and 85–100% for heparin plasma (n=5). Recovery ranges are 85–98% (average 91%) for serum, 87–101% (average 93%) for EDTA plasma, and 94–102% (average 98%) for heparin plasma.