Mouse Glucose 6 Phosphate Isomerase ELISA Kit

Assay Genie supplies a colorimetric Sandwich ELISA kit built on a double-antibody format for measuring glucose-6-phosphate isomerase in mouse-derived samples. The enzyme participates in glycolysis and gluconeogenesis, and its dysregulation has been linked to metabolic disorders, autoimmune disease, and cancer.

Stated analytical performance includes a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for 12 months.

Reported linearity for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions spans 80-105%. Recovery ranges are 88-101% for serum (average 94%), 87-96% for EDTA plasma (average 92%), and 86-103% for heparin plasma (average 92%). The protocol calls for equilibrating reagents and TMB substrate to room temperature, loading standards, samples and a zero control onto the pre-coated plate, incubating at 37°C for 90 minutes, adding biotin-labelled detection antibody with a 60-minute 37°C incubation, adding HRP-streptavidin for 30 minutes at 37°C, developing with TMB substrate in the dark for 10-20 minutes, and reading absorbance at 450 nm after adding stop solution.