Mouse Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit

G6PD (Glucose-6-phosphate dehydrogenase) catalyzes the rate-limiting step of the pentose phosphate pathway, generating NADPH that sustains reduced glutathione pools and shields cells from reactive oxygen species. Mouse models are routinely used to study how altered G6PD activity contributes to hemolytic anemia, neurodegeneration, metabolic syndrome, and malaria susceptibility, which makes a quantitative assay for this enzyme broadly useful across hematology, redox biology, and toxicology research.

The Assay Genie Mouse G6PD ELISA Kit provides an immunoassay-based method for measuring G6PD levels in murine samples, with results read as absorbance values and back-calculated against a kit-specific standard curve. It is supplied as a complete reagent set in a microplate format; the protocol supports processing of standards, controls, and unknowns in a single experimental run. Storage guidance calls for refrigeration of unopened components, equilibration to room temperature before each assay, and protection of working solutions from unnecessary freeze-thaw cycling.

Intended for research use only, the kit is suited to studies comparing wild-type and knock-out mouse strains, evaluating pharmacological modulators of the pentose phosphate pathway, and profiling tissue-derived samples in models of oxidative injury.