Mouse Glucagon PharmaGenie ELISA Kit (SBRS0055)

Quantitative measurement of mouse glucagon in cell culture supernatants and serum samples is enabled by this competitive ELISA from the Assay Genie PharmaGenie reagent line. Glucagon is a 29-amino acid peptide hormone secreted by pancreatic alpha cells that opposes insulin action and raises blood glucose, so reliable quantitation of circulating levels is foundational to diabetes, obesity, and broader metabolic disease research. The kit runs 96 determinations per microplate using an absorbance-based readout, allowing batch processing of treatment cohorts and time-course samples in studies of alpha-cell biology, insulin-glucagon axis dynamics, hypoglycemic counter-regulation, and drug-response profiling in murine models. The competitive format is a common choice for small peptides, which are often difficult to capture with conventional two-antibody assays. Serum sampling from fasted or glucose-challenged mice and conditioned media from isolated islets or alpha-cell lines are typical inputs. Each kit contains a 96-assay microplate and all reagents required to run the standard curve and unknown samples; standard laboratory absorbance equipment is sufficient for signal acquisition. Reagents are supplied for refrigerated storage as detailed in the kit insert, which should be reviewed before first use. For research use only.