Mouse Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit

This 96-assay immunoassay from Assay Genie enables quantitative measurement of glucagon-like peptide-1 receptor (GLP1R) in mouse tissue homogenates and cell lysates. The kit is configured for in vitro research workflows and includes all reagents required to run a full standard curve plus samples in duplicate on a single microplate.

GLP1R is a class B G-protein coupled receptor with a molecular mass of approximately 53 kDa that mediates incretin signaling downstream of GLP-1, a hormone central to glucose homeostasis, insulin secretion, and appetite regulation. The receptor is widely studied in diabetes, obesity, and metabolic disease research, with particular relevance to GLP-1 receptor agonist pharmacology and pancreatic beta-cell biology. Profiling GLP1R expression across genotypes, dietary conditions, or pharmacological treatments helps researchers characterize receptor-level changes that complement downstream signaling readouts.

Each kit is supplied with a pre-coated microplate, capture and detection reagents, calibrated standards, and assay buffer. The procedure follows a standard ELISA workflow compatible with benchtop plate readers. Reconstituted components should be handled per the enclosed insert, with working aliquots prepared to minimize freeze-thaw cycles of the antibody and standard preparations.

Recommended applications include basic and translational studies of incretin biology, evaluation of GLP1R expression in transgenic or knockout mouse models, and characterization of candidate therapeutics targeting the GLP-1 pathway. The assay is intended for research use only by laboratory personnel experienced with ELISA protocols and the handling of membrane-associated receptor proteins.