Mouse Gli1 (Zinc finger protein GLI1) ELISA KIT (MOFI01416)

The Hedgehog signaling pathway is a conserved regulatory cascade with broad relevance to developmental biology, stem cell maintenance, and oncology, and the zinc finger transcription factor Gli1 acts as its terminal effector. This two-antibody ELISA from Assay Genie quantifies endogenous mouse Gli1 protein using a colorimetric readout, supporting pathway-activity studies in rodent models of Hedgehog-driven disease. Researchers apply it to evaluate Gli1 expression in tumor xenografts, embryonic tissue lysates, and stem-cell differentiation time courses, and it integrates into inhibitor screens targeting Smoothened, PTCH1, or downstream Gli regulators. The kit ships in a 96 Assays configuration, validated for use with mouse-derived blood fractions, and is intended for research use only. Refrigerated storage is recommended to preserve antibody and standard stability; reagents should be protected from light and allowed to equilibrate before running the assay.