Mouse GFAP (glial fibrillary acidic protein) ELISA Kit (MOES01071)

Assay Genie offers this mouse GFAP (glial fibrillary acidic protein) ELISA kit for the in vitro quantitative determination of mouse GFAP. It uses a sandwich ELISA format, and the microplate is pre-coated with an antibody specific to mouse GFAP. The kit is intended for serum, plasma and other biological fluids, and the manufacturer also describes quantification in mouse serum, plasma and cell culture supernatants. The stated assay time is 3.5h.

Performance specifications list a detection range of 15.63-1000 pg/mL and a sensitivity of 9.38 pg/mL. Recovery is given as an average of 96% (90-101%) in serum, 95% (89-100%) in EDTA plasma and 98% (91-103%) in cell culture media. The kit shows no significant cross-reactivity or interference between mouse GFAP and analogues, and both intra-CV and inter-CV are stated to be < 10%.

GFAP is a class-III intermediate filament protein found in astrocytes, acting as a cell-specific marker that distinguishes astrocytes from other glial cells during central nervous system development. The manufacturer also notes that abnormal GFAP levels are associated with neurological disorders such as Alzheimer's disease, multiple sclerosis and brain injuries, and describes the kit as a tool for investigating the role of GFAP in neurobiology.