Mouse GC (Glucagon) ELISA Kit (MOES01072)

Assay Genie offers a colorimetric competitive ELISA for measuring GC (glucagon) in mouse serum, plasma and other biological fluids. The kit is described for measuring GC in serum, plasma, lysate samples and cell culture supernatants, and is listed for research in cancer, metabolism, developmental biology, signal transduction and stem cells. Glucagon is described as a hormone that stimulates the liver to release glucose into the bloodstream, with dysregulation associated with diabetes and metabolic disorders.

The stated sensitivity is 18.75 pg/mL, with a detection range of 31.25-2000 pg/mL and a sample volume of 50 μL. The assay time is 2 h 30 min, and the manufacturer reports recovery of 80%-120%. The kit recognizes mouse GC, with no significant cross-reactivity or interference observed between mouse GC and analogues.

Components include a dismountable microplate, reference standard, sample diluent, biotinylated detection antibody, HRP conjugate, wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Listed storage conditions include 2-8°C for diluents, wash buffer, substrate reagent and stop solution, with the microplate and reference standard also listed at -20°C. Listed synonyms for the analyte are GCG, GLP1, GLP2 and GRPP.