Mouse Gastric inhibitory polypeptide receptor (Gipr) ELISA Kit

Assay Genie supplies an ELISA Kit intended for the quantitative measurement of mouse Gastric inhibitory polypeptide receptor (GIPR) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay is reactive with mouse samples. Synonyms reported for the target include Glucose-dependent insulinotropic polypeptide receptor and GIP-R.

GIPR is described as a receptor for GIP, with activity mediated by G proteins that activate adenylyl cyclase. According to UniProt entry Q0P543, GIPR belongs to the G-protein coupled receptor 2 family, is located at the cell membrane as a multi-pass membrane protein, and has a reported molecular weight of 26,617 Da. The kit's stated specificity covers natural and recombinant mouse Gastric inhibitory polypeptide receptor, and the receptor is noted to possibly form homodimers and heterodimers with GLP1R. The listed research area is Neurosciences.

Reported performance characteristics from the manufacturer include a detection range of 0.625-40ng/mL, a sensitivity of 0.331ng/mL, an intra-assay CV of 6.9%, and an inter-assay CV of 8.8%. Linearity in serum (N=5) at dilutions 1:2, 1:4, 1:8 and 1:16 was reported across the ranges 104-116%, 99-109%, 86-98% and 102-111% respectively; EDTA plasma (N=5) values at the same dilutions spanned 104-113%, 83-83%, 97-107% and 111-120%; heparin plasma (N=5) values spanned 107-117%, 96-108%, 102-114% and 83-92%. Recovery in serum gave an average of 80% with a range of 80-86%, and in plasma an average of 82% with a range of 80-88%. The kit is shipped at +4°C, stored at -20°C, and is intended for research use only.