Mouse GAL12 (Galectin 12) ELISA Kit (MOES01054)

Galectin-12, encoded by the LGALS12 gene (also known as GRIP1), is a tandem-repeat lectin featuring two carbohydrate-recognition domains that bind β-galactosides. It is predominantly expressed in adipose tissue, co-localizes with Perilipin-1 on lipid droplet surfaces, and is also detected in macrophages and other leukocyte populations. The Assay Genie Mouse GAL12 ELISA Kit supports quantitative work in metabolic disease models, including 3T3-L1 preadipocyte differentiation studies where galectin-12 modulates C/EBPα, C/EBPβ, and PPARγ transcription factor expression. Researchers investigating obesity, insulin resistance, and chronic adipose tissue inflammation can apply this microplate format to track how galectin-12 regulates lipolysis through cyclic AMP and PKA signaling, and how its suppression in macrophages skews polarization toward the pro-inflammatory M1 phenotype.

After reconstitution, working standards should be aliquoted into single-use vials and frozen at -20°C to limit freeze-thaw degradation, while the pre-coated strips remain stable at 4°C when sealed. The biotinylated detection antibody and streptavidin-HRP conjugates in the supplied kit tolerate short-term refrigerated storage for routine use. Compatible biological matrices include serum, plasma, tissue homogenates, cell lysates, and cell culture supernates, and the assay has been validated to show no significant cross-reactivity with related galectin family members such as galectin-10. For research use only.