Mouse Fructose-bisphosphate aldolase B (Aldob) ELISA Kit

Assay Genie offers a sandwich-format ELISA kit for the quantitative measurement of mouse Fructose-bisphosphate aldolase B (ALDOB), an enzyme involved in glycolysis that plays a key role in energy production and metabolism. The kit is intended for research use only and supports studies in metabolism, where dysregulation of ALDOB has been linked to metabolic disorders and liver diseases, making it a valuable biomarker for these research areas.

According to the manufacturer datasheet, the assay detects natural and recombinant mouse Fructose-bisphosphate aldolase B and is validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reported performance characteristics include a detection range of 78-5000pg/mL, a sensitivity of 39pg/mL, an intra-assay CV of 5.4%, and an inter-assay CV of 8.1%. Linearity has been demonstrated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, and recovery data are provided for serum and plasma samples.

The kit uses a colorimetric detection method, with absorbance read at 450 nm, and supplies the components required for a complete sandwich ELISA workflow, including a dismountable microplate, lyophilized standard, sample and assay diluents, detection reagents, wash buffer, substrate, and stop solution. Storage conditions are specified per component in the kit insert.