Mouse Forkhead box protein O1 (Foxo1) ELISA Kit

Assay Genie provides a Sandwich ELISA Kit designed for the quantitative detection of mouse Forkhead box protein O1 (FOXO1). The kit is reactive with mouse samples and is intended for research use only, supporting studies into the role of FOXO1 in cellular processes such as cell cycle progression, apoptosis, and metabolism, as well as its dysregulation in diseases including diabetes, cancer, and neurodegenerative disorders.

The assay is run on a colorimetric detection platform and accepts serum, plasma, and tissue culture supernatants. According to the manufacturer, the detection range is 78-5000pg/mL with a sensitivity of 39.6pg/mL. Reported intra-assay and inter-assay CV values are both 0.0%. Linearity has been evaluated in serum (95-121% across dilutions), EDTA plasma (81-107%), and heparin plasma (95-115%), while recovery in both serum and plasma averages 80% (range 80-80%). The kit specifically recognises natural and recombinant mouse Forkhead box protein O1, and the target protein is catalogued under Uniprot Q9R1E0. Synonyms for the target include Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, Fkhr, and Foxo1a.

FOXO1 is a transcription factor described as the main target of insulin signalling, regulating metabolic homeostasis in response to oxidative stress and binding to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3'. It localises to the cytoplasm and nucleus, shuttling between these compartments, and is identified as a research-area target in cancer studies.