Mouse Follistatin (FS) ELISA Kit

Built around a competitive immunoassay workflow, this kit from Assay Genie quantifies mouse Follistatin (FS), a secreted glycoprotein best known for binding and neutralizing activin and other TGF-β superfamily ligands. Researchers studying skeletal muscle hypertrophy, folliculogenesis, gonadal feedback, hepatic injury, or inflammatory signaling use FS concentrations as a circulating biomarker of activin-axis activity.

The package is supplied as a complete 96 assays kit containing pre-coated wells, assay-specific standards, detection antibody, and substrate for a colorimetric end-point read on a standard microplate reader. Mouse serum samples are the primary validated input; heparin or EDTA plasma and cleared tissue-culture supernatant can also be run where the laboratory has confirmed dilution linearity for its own matrix.

The plate works on a competition principle: Follistatin present in the sample competes with coated FS antigen for binding to a labelled antibody, so absorbance falls as analyte concentration rises. The supplied calibrators define the standard curve used to convert those readings into quantitative values. Unopened components should be kept refrigerated and brought to room temperature before use to avoid condensation in the wells.