Mouse FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit (MOES00945)

The Assay Genie mouse FNDC5 immunoassay quantitatively measures fibronectin type III domain-containing protein 5, the transmembrane precursor of the myokine irisin, in murine research specimens. This colorimetric plate-format kit supports investigation of exercise physiology, brown adipose tissue thermogenesis, and metabolic disease by detecting circulating FNDC5 in samples collected from mouse models of obesity, insulin resistance, and physical activity interventions.

Each kit supplies pre-coated microwell strips, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards in sealed vials, and buffer reagents sufficient for 96 determinations. Researchers investigating UCP1-mediated browning of white adipose depots, PGC-1α signaling cascades, and whole-body energy expenditure can use the assay to quantify FNDC5 expression changes in response to treadmill training, cold exposure, or pharmacological treatment. The workflow accommodates standard absorbance plate readers and integrates into broader studies of muscle-adipose crosstalk, glucose homeostasis, and body weight regulation.