Mouse Fibrinogen ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative detection of fibrinogen in mouse samples. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with storage at 2-8°C for 12 months.

Fibrinogen is a key protein involved in blood clotting and inflammation, supporting research in cardiovascular disease, inflammation, and coagulation disorders. Reported performance includes linearity for EDTA plasma (87-105%, 83-99%, 82-88% at 1:2, 1:4, 1:8) and heparin plasma (91-104%, 87-101%, 82-100% across the same dilutions), with average recovery of 94% in EDTA plasma and 97% in heparin plasma. The kit includes a pre-coated dismountable microplate, lyophilized standard, biotin-labelled detection antibody, HRP-Streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, wash buffer, stop solution, plate sealer, and technical manual.

The assay workflow involves equilibrating reagents to room temperature, incubating standards and samples on the pre-coated plate at 37°C for 90 minutes, adding biotin-labelled detection antibody for a 60-minute incubation, adding HRP-Streptavidin for 30 minutes, developing colour with TMB substrate in the dark for 10-20 minutes, then adding stop solution and reading absorbance at 450 nm. Sample preparation guidance covers serum, plasma, tissue homogenate, cell culture supernatant, and cell lysate.