Mouse FGF9 ELISA Kit

Assay Genie's Mouse FGF9 ELISA Kit is a sandwich, double-antibody colorimetric assay designed for the quantitative measurement of fibroblast growth factor 9 in mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit supports research in developmental biology, where FGF9 is studied for its role in regulating cell proliferation, differentiation and embryonic development.

The assay has a sensitivity of 37.5 pg/mL and a standard curve range of 62.5–4000 pg/mL. Linearity was validated across serum, EDTA plasma and heparin plasma (n = 5) at dilutions of 1:2, 1:4 and 1:8, yielding serum values of 85–102%, 87–101% and 86–100%, EDTA plasma values of 86–103%, 82–99% and 87–95%, and heparin plasma values of 85–102%, 84–92% and 82–100%. Spike-and-recovery averages were 96% for serum (range 93–102%), 91% for EDTA plasma (range 86–99%) and 93% for heparin plasma (range 90–96%).

Unopened components are stored at 2–8°C and remain stable for 12 months. The assay targets FGF9 (UniProt P54130), also referred to by the synonyms FGF-9, GAF, HBFG-9, HBGF-9, MGC119914, MGC119915 and SYNS3.