Mouse FGF6 (Fibroblast Growth Factor 6) CLIA Kit (MOES00110)

FGF6, also referenced in the literature as HST-2 or HSTF-2, is a member of the heparin-binding FGF family that preferentially activates FGFR1, FGFR2, and FGFR4 isoforms expressed on myogenic progenitors. In adult mouse tissue, FGF6 transcripts concentrate in skeletal muscle and testis, and its upregulation is a hallmark of the early phase of muscle regeneration following cardiotoxin or crush injury.

The Assay Genie chemiluminescence workflow typically reaches a lower limit of quantification in the low pg/mL range, with a standard curve spanning roughly 7-8 logs when serially diluted. Recommended sample volume is 50-100 μL of serum or plasma per well, run in duplicate alongside an internal control to flag plate-to-plate variation. Reported intra-assay precision is typically <10% CV, with inter-assay CV below 15%.

Researchers studying Duchenne muscular dystrophy models (mdx mouse), aging-related sarcopenia, or exercise-induced muscle hypertrophy commonly pair this reagent with qPCR or western blot readouts of myogenic markers such as MyoD, Myogenin, and Pax7. The 96-well microplate format also supports medium-throughput screening of conditioned media from primary myoblast or C2C12 cultures.