Mouse FGF 23/Fibroblast Growth Factor 23 ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie designed for the precise quantitative measurement of fibroblast growth factor 23 (FGF-23) in mouse samples. The assay uses a double-antibody sandwich format with a colorimetric detection method and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a stated sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. The kit is intended for research applications relating to FGF-23's role as a regulator of phosphate and vitamin D metabolism and mineral homeostasis, supporting investigations into conditions such as chronic kidney disease, cardiovascular disorders, and bone disorders. The kit falls within the developmental biology research area and is supplied for storage at 2–8°C for up to 12 months.

The manufacturer reports linearity across dilutions (1:2, 1:4 and 1:8) for serum (88–104%, 83–97%, 87–96%), EDTA plasma (89–103%, 82–99%, 81–92%) and heparin plasma (87–103%, 83–97%, 92–98%), together with spike-and-recovery performance of 86–100% (average 93%) for serum, 86–97% (average 90%) for EDTA plasma and 85–103% (average 94%) for heparin plasma. The assay procedure involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes at 37°C, HRP-streptavidin binding for 30 minutes at 37°C, TMB colour development in the dark for 10–20 minutes, and a stop reaction read at 450 nm.