Mouse Fatty acid desaturase 3 (Fads3) ELISA Kit

Assay Genie supplies a Sandwich ELISA designed for the quantitative detection of mouse Fatty acid desaturase 3 (Fads3) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses a colorimetric detection method and is intended for research use only. FADS3 is described as a methyl-end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between a preexisting double bond and the terminal methyl group of the fatty acyl chain, desaturating (11E)-octadecenoate (trans-vaccenoate) at carbon 13 to generate (11E,13Z)-octadecadienoate and likely participating in the biohydrogenation pathway of linoleic acid (LA) (18:2n-6). The protein is localised to the endoplasmic reticulum membrane as a multi-pass membrane protein, and the kit is grouped under the Cardiovascular research area.

Performance characteristics quoted from the product specification include a detection range of 0.156–10 ng/ml, a sensitivity of 0.089 ng/mL, an intra-assay CV of 5.5%, and an inter-assay CV of 7.9%. Specificity is stated for natural and recombinant mouse Fatty acid desaturase 3. Reported spike-and-recovery values are an average of 88% (range 82–94%) for serum and 90% (range 84–96%) for plasma. Linearity data for serum, EDTA plasma and heparin plasma are provided at dilutions of 1:2, 1:4, 1:8 and 1:16 across five replicates. Synonyms for the target listed with the product include Delta(13) fatty acid desaturase, Delta(13) desaturase and MNCb-0629; the associated UniProt entry is Q9JJE7 and the protein molecular weight is reported as 51,469 Da.