Mouse Fatty acid-binding protein, brain (Fabp7) ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative detection of mouse Fatty acid-binding protein, brain (Fabp7). The kit is suitable for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and reacts with mouse Fabp7 (also known as BLBP, B-FABP, Brain lipid-binding protein, Brain-type fatty acid-binding protein, and Blbp), recognising both natural and recombinant forms of the target. Detection is by colorimetric readout measured at 450 nm.

Reported performance figures include a detection range of 1.56-100ng/mL, a sensitivity of 0.783ng/mL, an intra-assay CV of 4.9%, and an inter-assay CV of 7.1%. Recovery averages 95% (range 89-101%) in serum and 97% (range 91-103%) in plasma, and linearity has been evaluated at 1:2, 1:4, 1:8, and 1:16 dilutions in serum, EDTA plasma, and heparin plasma. The associated protein (UniProt P51880, NCBI Gene ID 12140, reported molecular weight 14,893 Da) is implicated in the transport of a hydrophobic ligand with morphogenic activity during CNS development, including a role in establishing the radial glial fiber system. The kit is listed for cardiovascular research applications and is for research use only.

Storage conditions vary by component: the microplate, standards, sample and assay diluents, and detection reagents are kept at -20°C, while wash buffer, substrate, and stop solution are stored at 4°C. Additional required materials include a microplate reader with a 450 nm filter, a multichannel pipette, an incubator, and deionised or distilled water.