Mouse Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

Fatty Acid Binding Protein 3 (H-FABP / MDGI), a small cytosolic carrier that shuttles long-chain fatty acids inside cardiac and skeletal myocytes and leaks rapidly into circulation after ischemic injury, can be quantitatively measured in mouse specimens with this colorimetric Assay Genie ELISA Kit, supplied as a microplate kit of 96 determinations for research use only. The assay is calibrated against native mouse FABP3 and is reactive with a panel of mouse-derived specimens, including tissue homogenates, whole-cell lysates, conditioned cell-culture media, and serum-based or plasma-based liquid samples.

Detection is by an enzymatic colorimetric reaction read on a standard microplate reader, with standards and unknowns typically run in duplicate to fit a standard curve and a full set of samples on the same plate. Unopened components are shipped refrigerated and should be stored refrigerated until first use; reconstituted or diluted working solutions are best preserved by freezing in small aliquots to limit freeze-thaw loss of activity.

FABP3 is widely used as a circulating biomarker in murine models of acute myocardial infarction, reperfusion injury, exercise-induced cardiac stress, metabolic syndrome, and skeletal-muscle disorders, making the kit useful for cardiovascular, metabolic, and exercise-physiology research programs.