Mouse Factor 10 (Factor X) ELISA Kit

Assay Genie provides a colorimetric sandwich ELISA, double antibody kit intended for the precise measurement of Factor 10 (Factor X) levels in mouse samples, including serum, plasma, cell culture supernatant, and cell or tissue lysate. Factor 10, also known as Factor X, is a vital component in the blood coagulation cascade, and its dysregulation has been linked to bleeding disorders or thrombotic events, making it a useful biomarker for coagulation-related research.

The assay has a reported sensitivity of 0.563 ng/mL and a measuring range of 0.938–60 ng/mL. Linearity in EDTA plasma (n=5) spans 84–100% across dilutions up to 1:8, while heparin plasma (n=5) shows 81–100% linearity. Recovery ranges from 85–96% (average 92%) in EDTA plasma and 86–102% (average 95%) in heparin plasma. The protocol uses a TMB substrate with absorbance read at 450 nm after stopping the reaction, and the kit should be stored at 2–8 °C for 12 months.

Synonyms for the target include FX, F10, Coagulation Factor X, Cf10, FXa, Prothrombinase, Stuart Factor, factor Xa, FXA, and Stuart-Prower factor. The kit supports research applications including the investigation of coagulation disorders, drug development, and personalised medicine approaches.