Mouse Fabp4 (Fatty acid-binding protein, adipocyte) ELISA Kit (MOFI01444)

Assay Genie's quantitative immunoassay for mouse fatty acid-binding protein 4 supports metabolic, adipose, and cardiometabolic research workflows. FABP4 (also called aP2 or ALBP) is a 15 kDa intracellular lipid chaperone secreted by adipocytes and macrophages, and circulating levels serve as a biomarker in studies of obesity, insulin resistance, type 2 diabetes, atherosclerosis, and non-alcoholic fatty liver disease.

The microplate-based kit is configured for research use only and is validated for use with mouse-derived liquid specimens, including serum or EDTA plasma, under standard in vitro laboratory conditions. The kit is supplied as a complete set with a pre-coated plate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate, and stop solution sufficient to run one full plate.

For storage, keep all components refrigerated and protected from light until first use, avoid repeated freeze-thaw cycles of reconstituted standards, and equilibrate reagents to room temperature before pipetting. Researchers commonly run a standard curve in duplicate alongside unknown samples, using a microplate reader to record absorbance values that are then interpolated against the standard curve to quantify FABP4 concentrations.