Mouse EPO/Erythropoietin ELISA Kit

Quantitative measurement of erythropoietin in murine serum-type samples, plasma preparations, and conditioned cell culture media is supported by this immunoassay from Assay Genie. The kit uses pre-coated microplate wells and a biotinylated detection antibody to capture circulating EPO, a glycoprotein hormone central to erythropoiesis and oxygen homeostasis. Investigators probing anemia models, polycythemia, hypoxia response pathways, and renal erythropoietin production depend on accurate quantification of this cytokine.

The standard curve spans 7.8 to 500 pg/mL, with a sensitivity near 2 pg/mL that enables detection of low endogenous circulating levels. A 50 uL sample volume is required per well, and the protocol completes in roughly four hours. Cross-reactivity with rat EPO is reported as minimal, while no significant cross-reactivity is observed with human erythropoietin, supporting species-specific interpretation in mouse studies.

Each kit includes a 96-well strip plate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate solution, and stop reagent. Store all components refrigerated at 4°C, protect from light, and aliquot reconstituted standards before freezing for repeated use. For research use only; not for diagnostic procedures.