Mouse Epiregulin (EREG) ELISA Kit

Epiregulin is a heparin-binding member of the EGF growth factor family that activates ErbB1 (EGFR) and ErbB4 receptors, and quantitative measurement of this ligand in murine specimens supports studies of mucosal barrier repair, keratinocyte proliferation, dermal inflammation, colitis, fibrosis, and tumor-microenvironment crosstalk. This mouse-reactive immunoassay kit from Assay Genie is configured on a microplate format calibrated against a recombinant mouse EREG standard curve, allowing laboratories to evaluate endogenous ligand release from cultured cells, tissue homogenates, and other sample matrices collected in murine studies. Each pack contains pre-coated wells, a matched detection antibody concentrate, standard reconstitution buffer, wash concentrate, and chromogenic substrate sufficient for one full plate run, with reagents validated for research use only. Protocols are written for use alongside complementary techniques such as western blot, qPCR, flow cytometry, and immunohistochemistry that probe the same ligand-receptor axis at the gene or surface-expression level. Researchers investigating amphiregulin and TGF-alpha cross-talk, goblet cell restitution following injury, or EGFR-driven epithelial remodeling in models of dermatitis will find this reagent a practical adjunct to in vivo and in vitro workflows. Refrigerated storage preserves kit components through the stated shelf life, and reconstituted standards remain stable through multiple freeze-thaw cycles when aliquoted on receipt. The complete pack is supplied as one microplate kit with a printed protocol booklet covering standard-curve preparation, sample-handling guidance, and four-parameter logistic data reduction.