Mouse Ephb4/Ephrin type-B receptor 4 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit provides quantitative detection of Ephrin type-B receptor 4 (EphB4) in mouse samples. The kit uses a double-antibody sandwich format with colorimetric detection and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The assay achieves a sensitivity of 18.75 pg/mL with a working range of 31.25-2000 pg/mL. Reported recovery averages 97% in serum, 96% in EDTA plasma, and 97% in heparin plasma, with linearity ranges of 85-104% (serum), 82-101% (EDTA plasma), and 80-99% (heparin plasma) across dilution ratios. The associated research area is cardiovascular, and the protein reference is Uniprot P54761.

Reagents should be stored at 2-8°C for 12 months. The kit includes a dismountable ELISA microplate, lyophilized standard, biotin-labeled detection antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual.