Mouse Eotaxin-1 PharmaGenie ELISA Kit (SBRS1347)

The Mouse Eotaxin-1 ELISA Kit from Assay Genie provides quantitative measurement of CCL11 (Eotaxin-1), a small ~8-9 kDa C-C chemokine that drives eosinophil recruitment in allergic inflammation. Researchers use this kit to investigate eosinophil trafficking in mouse models of asthma, atopic dermatitis, and helminth infection, and to evaluate anti-inflammatory compounds targeting the CCR3-Eotaxin axis. The protocol accommodates cell culture supernatant and plasma biospecimens, with the Assay Genie microplate pre-coated with anti-mouse CCL11 capture antibody and a biotinylated detection antibody pair. Each kit includes lyophilized recombinant standards, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer, and plate sealers for one full microplate run. Reconstituted standards and detection antibody should be stored at -20°C in single-use aliquots to limit freeze-thaw loss, while the coated plate and remaining kit components are kept refrigerated at 4°C until first use. This product is intended for research use only in laboratory settings and is not validated for diagnostic procedures.