Mouse Enhancer of filamentation 1 (Nedd9) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the accurate quantification of Mouse Enhancer of filamentation 1 (Nedd9) levels in mouse samples. The kit is reactive with mouse and accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, using a colorimetric detection method.

Manufacturer specifications list a detection range of 0.156-10ng/mL and a sensitivity of 0.064ng/mL, with specificity for natural and recombinant mouse Enhancer of filamentation 1. Nedd9, also referred to as HEF1, CAS-L, NEDD-9, p105, mEF1, Casl or CRK-associated substrate-related protein, is described as a docking protein that coordinates tyrosine-kinase-based signalling related to cell adhesion. The target is implicated in cell migration, adhesion and invasion processes, supporting its use as a biomarker within cancer research.

Associated with UniProt entry O35177 and a reported molecular weight of 93,052 Da, the target localises to the cytoplasm, cell cortex, nucleus and cytoskeleton. Reported linearity across dilutions from 1:2 to 1:16 covers 96-106%, 112-122%, 82-92% and 80-89% for serum; 90-98%, 89-99%, 95-105% and 104-114% for EDTA plasma; and 97-107%, 91-101%, 110-120% and 104-113% for heparin plasma. For research use only.