Mouse Elongation of very long chain fatty acids protein 1 (Elovl1) ELISA Kit

This colorimetric sandwich ELISA kit from Assay Genie is designed for the quantitative detection of mouse Elongation of very long chain fatty acids protein 1 (Elovl1) in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The assay is reactive with mouse samples and is specific for natural and recombinant mouse Elovl1. Synonyms for the target include 3-keto acyl-CoA synthase Elovl1, ELOVL fatty acid elongase 1, very long chain 3-ketoacyl-CoA synthase 1, very long chain 3-oxoacyl-CoA synthase 1, ELOVL FA elongase 1, and Ssc1.

Performance characteristics include a detection range of 0.156-10ng/mL, a sensitivity of 0.081ng/mL, an intra-assay CV of 7.4%, and an inter-assay CV of 10.0%. Linearity has been validated across serial dilutions from 1:2 to 1:16 in serum (99-109% to 93-105%), EDTA plasma (93-103% to 111-121%), and heparin plasma (95-107% to 98-110%). Recovery is 80-87% (average 81%) for serum and 80-89% (average 83%) for plasma.

Elovl1 is a condensing enzyme that localises to the endoplasmic reticulum membrane as a multi-pass protein and catalyses the first, rate-limiting reaction of the long-chain fatty acid elongation cycle, adding two carbons per cycle to produce very long chain fatty acids (VLCFAs). It exhibits activity toward saturated C18 to C26 acyl-CoA substrates, with the highest activity towards C22:0 acyl-CoA, and is important for saturated C24:0 and monounsaturated C24:1 sphingolipid synthesis. The protein interacts with LASS2, TECR, and HSD17B12, has a molecular weight of 32,678 Da (UniProt: Q9JLJ5), and the kit is intended for research use only.