Mouse ELN (Elastin) CLIA Kit (MOES00237)

Each kit provides pre-coated microplate wells, lyophilized standards, a detection antibody conjugate, and chemiluminescent substrate sufficient for 96 determinations run in duplicate. The sandwich immunoassay pairs a capture antibody specific to mouse elastin with a labeled secondary reporter, and signal is generated through an HRP–luminol reaction recorded in relative light units (RLU). Standard curves are typically constructed across seven points spanning approximately 31.2–2000 pg/mL, with an analytical sensitivity near 10 pg/mL, enabling reliable quantification in dilute serum, bronchoalveolar lavage fluid (BALF), and clarified tissue homogenates.

Assay Genie customers have applied this CLIA Kit to elastin remodeling studies in ApoE-knockout and angiotensin II infusion models of vascular stiffening, bleomycin- and elastase-induced pulmonary injury, and dermal photoaging or wound-healing paradigms. To limit matrix effects, serum and plasma are commonly diluted 1:2 to 1:10 in working buffer before loading, while BALF is usually run neat. Bring every component to room temperature before initiating the assay, aliquot the standard to prevent repeated freeze-thaw cycles, and avoid exposing the substrate to direct light to preserve chemiluminescent output.