Mouse ELMO2 (Engulfment And Cell Motility 2) ELISA Kit (MOES00991)

The Assay Genie Mouse ELMO2 ELISA Kit supports quantitative measurement of Engulfment and Cell Motility protein 2, a cytoplasmic scaffolding factor that cooperates with DOCK180-family Rho-GEFs to drive Rac1-dependent cytoskeletal remodelling, phagocytic cup formation, and directed cell migration. Researchers studying integrin signalling, leukocyte chemotaxis, vascular sprouting angiogenesis, and tumour cell invasion rely on this kit to evaluate phagocytic competence, vascular integrity in retinal and endothelial models, and Rac activation in knockout versus wild-type cohorts. Each shipment includes a pre-coated microplate, lyophilised standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent in individual vials. Reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles, while working dilutions of detection antibody and streptavidin-HRP remain stable refrigerated for the short term. The two-antibody immunoassay workflow immobilises a capture antibody on the plate and detects bound analyte with an enzyme-linked secondary reagent; for research use only, refer to the lot-specific certificate of analysis for the exact detection range, sensitivity, and recommended sample volume before running unknown specimens.