Mouse EGR2 (Early Growth Response Protein 2) ELISA Kit (MOES00974)

For quantitative measurement of mouse Early Growth Response Protein 2 (EGR2), also known as Krox-20, this immunoassay kit from Assay Genie provides a complete set of pre-coated reagents for high-throughput screening. EGR2 is a zinc-finger transcription factor critical for Schwann cell myelination, peripheral nerve development, and the differentiation of specific T-cell subsets, making accurate quantification valuable in neuroscience, immunology, and developmental biology research.

The kit uses an absorbance-based detection method on a microplate format, with sufficient reagents for 96 individual determinations. All required buffers, recombinant standards, detection antibody, and chromogenic substrate are supplied in ready-to-use vials, allowing researchers to generate a standard curve and measure endogenous EGR2 levels in cell lysates and tissue homogenates.

For optimal reagent stability, store unopened components refrigerated and aliquot the standard after reconstitution to avoid repeated freeze-thaw cycles. Reconstituted detection antibody should be kept at 4°C if used within the same week. The assay is calibrated specifically against the mouse EGR2 sequence and is intended for in vitro research use only, supporting applications such as western blot validation, flow cytometry confirmation, and gene-expression studies linking EGR2 activity to myelination disorders, Charcot-Marie-Tooth disease models, and autoimmune disease research.