Mouse Egl Nine Homolog 1 (EGLN1) ELISA Kit

Prolyl hydroxylase domain protein 2 (PHD2), the protein product of the Egl Nine Homolog 1 (EGLN1) gene, acts as a primary cellular oxygen sensor by hydroxylating specific proline residues on HIF-alpha subunits, marking them for VHL-mediated proteolysis under normoxic conditions. This Assay Genie immunoassay supports in vitro research by providing a quantitative tool to measure endogenous EGLN1 protein levels in mouse-derived samples, with applications spanning hypoxia signaling, ischemic disease models, tumor angiogenesis, and erythropoiesis regulation.

The kit uses an absorbance-based readout compatible with standard plate readers and is validated for tissue homogenates and cell lysates. Each pack contains reagents sufficient for 96 reactions. Investigators studying HIF pathway regulation, cellular oxygen sensing mechanisms, or EGLN1-linked disease models can apply this kit to track expression changes in response to genetic, pharmacological, or environmental manipulations in mouse model systems.