Mouse Ear 14/EosinophILassociated, ribonuclease A family, member 14 ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative detection of Eosinophil-Associated Ribonuclease A Family Member 14 (Ear 14) in mouse samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric readout and reports a sensitivity of 0.234 ng/mL, with a working range of 0.391–25 ng/mL.

Ear 14 is described as a protein involved in immune response and inflammation, with relevance to conditions such as allergies, asthma, and autoimmune diseases. Reported linearity across 1:2, 1:4, and 1:8 dilutions spans 86–105% for serum, 82–101% for EDTA plasma, and 80–100% for heparin plasma. Average recovery is 90% for serum, 93% for EDTA plasma, and 95% for heparin plasma. The protocol involves primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB colour development for 10–20 minutes in the dark, and absorbance reading at 450 nm. Kit reagents are stored at 2–8°C for 12 months.