Mouse Dystrophin ELISA Kit

From Assay Genie, this Sandwich ELISA, Double Antibody kit is designed for the quantitative measurement of dystrophin in mouse samples. The kit is described as offering high sensitivity and specificity for precise and reproducible results. Dystrophin is a critical protein involved in maintaining the structural integrity of muscle fibers, and its deficiency is associated with muscular dystrophy, making this kit relevant for studying disease progression and evaluating potential treatments.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 0.188 ng/mL and a detection range of 0.313–20 ng/mL, with colorimetric detection via TMB substrate and absorbance measurement at 450 nm. Reagents are stored at 2–8°C and are stable for 12 months. The associated UniProt entry is P11531, and the gene symbol is DMD.

Reported linearity across serum, EDTA plasma, and heparin plasma (n=5) at 1:2, 1:4, and 1:8 dilutions ranges from 83% to 105%. Recovery ranges fall between 85% and 104%, with average recovery between 93% and 94% depending on the sample matrix.