Mouse Doublecortin (DCX) ELISA Kit

Quantify mouse Doublecortin (DCX) with this ELISA Kit from Assay Genie, calibrated for research on neurogenesis and brain development. Doublecortin is a microtubule-associated phosphoprotein expressed by migrating neuroblasts and immature neurons, commonly used as a biomarker of adult hippocampal neurogenesis and neural stem cell differentiation.

This research-use-only kit is reactive specifically with mouse samples and supports analysis of tissue homogenates and cell lysates, enabling researchers to measure DCX expression in brain tissue extracts and cultured neuronal cells. The 96-assay microplate format processes multiple samples in parallel for comparative studies across experimental conditions.

Typical applications include tracking neurogenesis following pharmacological treatment, characterizing transgenic mouse models of neurological disease, and profiling DCX levels during development or after brain injury. Researchers investigating Alzheimer's disease, depression models, exercise-induced neurogenesis, and stroke recovery frequently rely on DCX quantification to assess new neuron formation.

Reagents should be refrigerated upon arrival and equilibrated to room temperature before use. The kit provides pre-coated microplates, buffers, and standards to perform the assay per the manufacturer's protocol. Absorbance readings are compared against the supplied standard curve to determine DCX concentrations in each sample.