Mouse DNA fragmentation factor subunit beta (Dffb) ELISA Kit

A Sandwich ELISA kit from Assay Genie designed for the precise measurement of DNA fragmentation factor subunit beta (Dffb) levels in mouse samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The kit is reactive with mouse samples and shows specificity for natural and recombinant mouse DNA fragmentation factor subunit beta.

Performance specifications include a detection range of 0.156-10ng/mL and a sensitivity of 0.086ng/mL. DFFB functions as a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis, where it acts as a heterodimer with DFFA. The protein is also known by the synonyms Caspase-activated deoxyribonuclease, DNA fragmentation factor 40 kDa subunit, CAD, DFF-40, and Cad, and is catalogued under UniProt O54788 with a molecular weight of 40 kDa. It is localised to the cytoplasm and nucleus.

Targeted primarily at cancer research, the kit contains standard sandwich ELISA components including a dismountable microplate, lyophilised standard, sample and assay diluents, detection reagents, wash buffer, substrate, stop solution, and plate sealer. The kit is for research use only.