Mouse DNA-binding protein Ikaros (Ikzf1) ELISA Kit

Designed for the accurate detection of DNA-binding protein Ikaros (IKZF1) levels in mouse samples, this Sandwich ELISA Kit from Assay Genie uses a colorimetric detection method in a solid-phase format and is applicable to serum, plasma, cell culture supernates, tissue homogenates and other biological fluids (C0; S1; S3). Specificity is reported for natural and recombinant mouse DNA-binding protein Ikaros, with synonyms listed as Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1, Ikaros, Lyf1, Zfpn1a1 and Znfn1a1 (S1).

Performance characteristics quoted by the manufacturer include a detection range of 0.156-10 ng/mL, a sensitivity of 0.085 ng/mL, an intra-assay CV of 8.9% and an inter-assay CV of 11.1% (S1). Linearity has been evaluated across serum, EDTA plasma and heparin plasma at dilutions of 1:2, 1:4, 1:8 and 1:16, with recovery in serum averaging 100% (range 94-106%) and in plasma averaging 102% (range 96-108%) (S1).

IKZF1 is described by the manufacturer as a key transcription factor involved in the regulation of gene expression and immune response, playing roles in lymphocyte development and chromatin remodelling via NuRD and BAF complexes, and being associated with autoimmune disorders and leukaemia (S1). The kit is intended for research use only, is classified under the Cancer research area, and the components require storage between -20°C and 4°C depending on the item, with international shipping at +4°C (S1; S3).