Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit

Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the terminal acylation step that converts diacylglycerol into triacylglycerol, and its expression in mouse tissues is a frequent target in studies of hepatic lipid accumulation, adipogenesis, dietary fat handling, and metabolic disease models. An immunoassay that reliably resolves DGAT1 abundance across serum samples, plasma draws, and tissue homogenates lets laboratories connect enzyme levels to phenotypes observed in knockout or diet-challenged cohorts.

This 96-assay colorimetric kit from Assay Genie is calibrated for mouse reactivity and includes the pre-coated microplate, lyophilized reference standards, biotinylated detection reagent, wash concentrate, substrate, and stop solution needed to run a complete batch. The protocol is written for in vitro research use, accommodates standard curve and unknown samples on the same plate, and is intended for refrigerated storage to preserve reagent activity until first use.

Typical applications include profiling DGAT1 expression in liver, adipose, and small-intestine lysates, screening pharmacological or genetic interventions that alter triglyceride synthesis, and supporting broader investigations into very-low-density lipoprotein secretion and steatohepatitis. Because the assay accepts multiple sample matrices from the same animal, it integrates readily into panel-style studies that also measure circulating lipids or glucose.