Mouse Defensin Beta 3 (Defb3) ELISA Kit

Quantitative ELISA reagent from Assay Genie for measuring murine Defensin Beta 3 (Defb3), also designated mBD-3, in laboratory research samples. Defb3 is a member of the beta-defensin family of small antimicrobial peptides involved in innate mucosal immunity and epithelial host defense in mice. This kit supports studies investigating antimicrobial peptide regulation, skin and mucosal barrier function, inflammatory responses, and bacterial challenge models in murine systems.

The assay is supplied in a microplate format sufficient for 96 determinations, with all required reagents included. Refrigerated storage preserves reagent stability; avoid repeated freeze-thaw cycles of prepared aliquots. Standards and detection antibody are provided in labeled vials, and the protocol accommodates small sample volumes consistent with murine specimen collection.

Applications include characterization of Defb3 expression in knockout and wild-type mouse strains, evaluation of antimicrobial peptide responses during infection challenge, and investigation of epithelial defense mechanisms. The kit is intended for research use only and is not validated for diagnostic or therapeutic applications.