Mouse DEFb14 (beta-defensin 14) ELISA Kit (MOES01768)

For investigators studying mucosal immunity and antimicrobial peptide biology, this Assay Genie kit provides quantitative measurement of mouse beta-defensin 14, a small cationic peptide expressed in the epididymis and other epithelial tissues where it contributes to innate defense and reproductive tract homeostasis. The 96-assay microplate format uses standard curve-based quantification with absorbance readout, suitable for serum and tissue homogenate samples collected from mouse infection or inflammation models. Each kit ships with pre-coated plate wells, lyophilized standard, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution, with reagents stored refrigerated at 4°C upon arrival and brought to room temperature before use. Unopened kit components remain stable through the printed expiration when held at the temperature indicated on each vial label, while working dilutions of standard and detection antibody are prepared fresh per assay. Defensin 14 expression is tracked in studies of urinary tract infection, prostatitis, epithelial barrier integrity, and comparative host defense across knockout strains, complementing transcript-level work with a protein readout. Reconstituted standard is aliquoted and frozen for repeat use, avoiding repeated freeze-thaw cycles that can degrade peptide standards. The kit is intended for research use only and is supplied with a lot-specific certificate and full protocol detailing recommended sample dilution, incubation times, and data analysis. Researchers should run all samples in duplicate with appropriate matrix-matched standard curves.