Mouse DEFA1/Defensin Alpha 1, Neutrophil ELISA Kit

Designed for quantitative measurement of mouse DEFA1 (Defensin alpha-1), this Sandwich ELISA from Assay Genie uses a double-antibody format with colorimetric detection to deliver sensitive and specific results. The kit is intended for research use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is reactive with mouse specimens. It has a reported sensitivity of 0.234 ng/mL and a working range of 0.391-25 ng/mL, with storage recommended at 2-8°C for 12 months.

DEFA1 is described as a key component of innate immunity, recognised for its antimicrobial activity and its role in the body's defence against pathogens. Research also implicates the protein in inflammation and broader immune responses, making it a useful biomarker for studies of infectious disease, autoimmune disorders, and inflammatory conditions. The kit is positioned as a versatile tool for these applications, offering reliable and reproducible measurement of DEFA1 across a variety of research settings.

Validation data provided with the product includes linearity measurements in serum, EDTA plasma, and heparin plasma, alongside recovery data for the same matrices. The assay procedure involves sequential incubation steps with a biotin-labelled detection antibody and HRP-Streptavidin conjugate, followed by TMB substrate for colour development with absorbance read at 450 nm.