Mouse DAN PharmaGenie ELISA Kit (SBRS1331)

DAN (Differential screening-selected gene Aberrant in Neuroblastoma), also known as NBL1, is a secreted glycoprotein belonging to the CAN family of BMP antagonists. This mouse-specific quantitative immunoassay from Assay Genie measures DAN levels in cell culture supernatants, plasma, or serum using a colorimetric readout. Researchers investigating bone morphogenetic protein signaling, neuroblastoma biology, and developmental patterning rely on this kit to characterize DAN's role as a secreted BMP inhibitor involved in cell differentiation and tumor suppression pathways.

The 96-assay microplate format provides ample wells for standard curve replicates and multiple sample conditions in a single run, supporting longitudinal studies of DAN expression in murine models. Each kit includes pre-coated plates and reagents to generate a standard curve from supplied calibrators, and is configured for research use only.

For sample preparation, blood-derived fractions (plasma or serum) should be separated promptly and stored refrigerated for short-term use, or frozen for longer-term analysis, while cell culture supernatants are typically clarified by centrifugation before running the assay.