Mouse D-Dimer ELISA Kit

A colorimetric Sandwich ELISA Kit from Assay Genie, configured as a Double Antibody assay, designed for the precise measurement of D-Dimer levels in mouse samples. The kit is intended for research applications, including studies of thrombotic disorders, where D-Dimer is a key biomarker indicating blood clot formation and degradation in the body.

According to the manufacturer, the assay has a sensitivity of 4.688 ng/mL and a measurable range of 7.813-500 ng/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for 12 months. Reported performance data include serum recovery averaging 98%, EDTA plasma averaging 94%, and heparin plasma averaging 91%, while linearity values across 1:2, 1:4, and 1:8 dilutions span 80-105%.

The assay procedure involves primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, and TMB color development for 10-20 minutes, with absorbance read at 450 nm after addition of stop solution.