Mouse D (2)dopamine receptor ELISA Kit

Designed for the precise measurement of D2 dopamine receptor levels in mouse samples, this Sandwich ELISA from Assay Genie uses a double-antibody, colorimetric detection format. The kit is compatible with serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, and is intended for research applications in neuroscience and drug development, including studies relevant to Parkinson's disease, schizophrenia, and addiction.

Reported sensitivity is 0.188 ng/mL with an assay range of 0.313-20 ng/mL. Unopened kits should be stored at 2-8°C for 12 months. The procedure involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after adding stop solution.

Reported linearity at 1:2 dilution (n=5) spans 86-105% for serum, 92-105% for EDTA plasma, and 88-104% for heparin plasma, with corresponding average recovery values of 100% (serum), 92% (EDTA plasma) and 94% (heparin plasma). The target is referenced under synonyms including Drd2, D2R, DRD2, Dopamine Receptor D2, and Dopamine D2 receptor, with Uniprot accession P61168.