Mouse Cytochrome P450 7A1 (CYP7A1) ELISA Kit

CYP7A1 (Cytochrome P450 7A1) is the rate-limiting hepatic enzyme in the classical bile acid synthesis pathway, catalyzing the 7α-hydroxylation of cholesterol. It serves as a key readout in studies of cholesterol homeostasis, hepatic lipid metabolism, and metabolic disease research, including atherosclerosis, non-alcoholic fatty liver disease, and bile acid signaling through FXR and TGR5 receptors.

The Assay Genie kit uses mouse-specific antibodies to quantitatively measure CYP7A1 in mouse-derived samples, including liver tissue homogenates, supporting cholesterol turnover studies in murine models. Standard curve-based quantitation enables comparison of enzyme levels across experimental cohorts, such as diet-induced, knock-out, or pharmacologically treated groups.

For reliable results, keep kit components refrigerated, protect from light, and avoid repeated freeze-thaw cycles of reconstituted reagents. Bring all reagents to room temperature before initiating the assay.