Mouse Cytochrome P450 1A2 (Cyp1a2) ELISA Kit

Assay Genie provides a colorimetric Sandwich ELISA Kit for the quantitative measurement of mouse Cytochrome P450 1A2 (CYP1A2), an important enzyme involved in the metabolism of various drugs, carcinogens and environmental toxins. The kit is designed for the accurate detection of CYP1A2 levels in mouse serum, plasma and cell culture supernatants, and is additionally compatible with tissue homogenates and other biological fluids.

Reported performance characteristics include a detection range of 0.312-20ng/mL and a sensitivity of 0.156ng/mL. Intra-assay precision is 7.0% and inter-assay precision is 9.4%. The assay is specific for natural and recombinant mouse Cytochrome P450 1A2, is classified under cardiovascular research, and is intended for research use only.

Cytochrome P450 1A2 is a heme-thiolate monooxygenase that, via NADPH-derived electrons, inserts one oxygen atom into a substrate. It catalyses the hydroxylation of endogenous substrates including fatty acids, steroid hormones and vitamins, and participates in the oxidative metabolism of xenobiotics, notably caffeine via N3-demethylation and the O-deethylation of phenacetin. Its activity, which can be influenced by factors such as diet, age and genetic variability, makes it a useful biomarker for drug metabolism, toxicology and pharmacokinetics in preclinical research.