Mouse Cytochrome P450 11A1 (CYP11A1) ELISA Kit

This quantitative immunoassay from Assay Genie measures mouse Cytochrome P450 11A1, the mitochondrial cholesterol side-chain cleavage enzyme (P450scc) that initiates steroidogenesis by converting cholesterol to pregnenolone. Researchers studying adrenal cortex function, gonadal steroid production, or placental hormone synthesis use this microplate kit, supplied in a 96-assay configuration, to quantify CYP11A1 protein levels in tissue homogenates and other biological samples prepared from murine sources.

The assay is appropriate for experiments profiling steroidogenic capacity across mouse tissues, characterising knockout or transgenic phenotypes affecting the hypothalamic-pituitary-gonadal or hypothalamic-pituitary-adrenal axes, and tracking enzyme expression changes in response to hormonal, pharmacological, or dietary interventions in vivo. Its reactivity is limited to mouse samples, making it a focused tool for preclinical rodent studies rather than cross-species screening.

Each kit provides pre-coated wells, assay reagents, and standard materials sufficient for 96 independent determinations. The workflow follows standard absorbance-based ELISA procedures compatible with routine microplate readers, and the kit is intended for research use only in laboratory settings studying reproductive biology, endocrinology, or mitochondrial enzyme regulation.