Mouse Cytochrome c oxidase subunit 5A, mitochondrial (Cox5a) ELISA Kit

A Sandwich ELISA kit from Assay Genie designed for the quantitative measurement of mouse Cytochrome c oxidase subunit 5A, mitochondrial (Cox5a). The kit uses a colorimetric detection method and is intended for use with mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay detects the target across a detection range of 0.312-20ng/mL with a sensitivity of 0.167ng/mL.

Reported performance characteristics include an intra-assay CV of 6.8% and an inter-assay CV of 8.1%. Linearity in serum (N=5) ranges from 86-98% to 109-119% across dilution factors 1:2 to 1:16; corresponding ranges are 120-120% to 83-95% for EDTA plasma and 84-93% to 100-110% for heparin plasma. Average recovery is 94% (range 88-100%) for serum and 96% (range 90-102%) for plasma. COX5A, also known by the synonym cytochrome c oxidase polypeptide Va, is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport, located on the mitochondrion inner membrane.

Specificity covers natural and recombinant mouse cytochrome c oxidase subunit 5A, mitochondrial (UniProt P12787; reported molecular weight 16,101 Da; NCBI Gene ID 12858). The kit is listed for research use only, with the indicated research area being Neurosciences.